| Rank | Team | Losses | Record |
| 1. | Brooklyn Dodgers, 1952 | 37 | 117-37 |
| 2. | Montreal Expos, 1994 | 37 | 125-37 |
| 3. | Boston Red Sox, 1948 | 42 | 112-42 |
| 4. | Chicago Cubs, 1945 | 44 | 110-44 |
| 5. | Montreal Expos, 1980 | 44 | 118-44 |
| 6. | Chicago White Sox, 1953 | 46 | 108-46 |
| 7. | New York Yankees, 1950 | 49 | 105-49 |
| 8. | Minnesota Twins, 1969 | 49 | 113-49 |
| 9. | Cincinnati Reds, 1976 | 49 | 113-49 |
| 10. | Montreal Expos, 1993 | 49 | 113-49 |
| 11. | Atlanta Braves, 1998 | 49 | 113-49 |
| 12. | Brooklyn Dodgers, 1953 | 50 | 104-50 |
| 13. | Seattle Mariners, 2001 | 50 | 112-50 |
| 14. | San Diego Padres, 2007 | 50 | 112-50 |
| 15. | Detroit Tigers, 1946 | 51 | 104-51 |
| 16. | Kansas City Royals, 1977 | 51 | 111-51 |
| 17. | Brooklyn Dodgers, 1941 | 52 | 102-52 |
| 18. | Boston Red Sox, 1942 | 52 | 102-52 |
| 19. | Brooklyn Dodgers, 1942 | 52 | 102-52 |
| 20. | St. Louis Cardinals, 1943 | 52 | 102-52 |
| 21. | St. Louis Cardinals, 1944 | 52 | 102-52 |
| 22. | Boston Red Sox, 1946 | 52 | 103-52 |
| 23. | Chicago Cubs, 1959 | 52 | 102-52 |
| 24. | San Francisco Giants, 1972 | 52 | 110-52 |
| 25. | Boston Red Sox, 1977 | 52 | 110-52 |
| 26. | Seattle Mariners, 1997 | 52 | 110-52 |
| 27. | Detroit Tigers, 1934 | 53 | 101-53 |
| 28. | Boston Red Sox, 1941 | 53 | 101-53 |
| 29. | Chicago White Sox, 1952 | 53 | 101-53 |
| 30. | New York Yankees, 1956 | 53 | 101-53 |
| 31. | Detroit Tigers, 1961 | 53 | 101-53 |
| 32. | Montreal Expos, 1974 | 53 | 109-53 |
| 33. | Boston Red Sox, 1986 | 53 | 109-53 |
| 34. | Seattle Mariners, 1991 | 53 | 109-53 |
| 35. | Boston Red Sox, 2011 | 53 | 109-53 |
| 36. | New York Yankees, 1939 | 54 | 100-54 |
| 37. | New York Yankees, 1958 | 54 | 100-54 |
| 38. | Los Angeles Dodgers, 1973 | 54 | 109-54 |
| 39. | Boston Red Sox, 1976 | 54 | 108-54 |
| 40. | Boston Red Sox, 1978 | 54 | 108-54 |
| 41. | Montreal Expos, 1982 | 54 | 108-54 |
| 42. | New York Mets, 1986 | 54 | 108-54 |
| 43. | New York Yankees, 2005 | 54 | 108-54 |
| 44. | Minnesota Twins, 2009 | 54 | 108-54 |
| 45. | New York Giants, 1938 | 55 | 99-55 |
| 46. | Brooklyn Dodgers, 1949 | 55 | 99-55 |
| 47. | San Francisco Giants, 1962 | 55 | 107-55 |
| 48. | San Francisco Giants, 1963 | 55 | 107-55 |
| 49. | San Francisco Giants, 1970 | 55 | 107-55 |
| 50. | Cincinnati Reds, 1973 | 55 | 108-55 |