| Rank | Team | Losses | Record |
| 1. | Seattle Mariners, 2010 | 122 | 40-122 |
| 2. | Chicago Cubs, 1981 | 121 | 41-121 |
| 3. | Seattle Mariners, 2005 | 120 | 42-120 |
| 4. | Kansas City Royals, 1969 | 118 | 44-118 |
| 5. | Detroit Tigers, 1975 | 115 | 47-115 |
| 6. | Oakland A's, 1977 | 115 | 47-115 |
| 7. | Milwaukee Brewers, 2009 | 115 | 47-115 |
| 8. | Seattle Mariners, 1983 | 114 | 48-114 |
| 9. | Washington Nationals, 2007 | 114 | 48-114 |
| 10. | Washington Nationals, 2008 | 114 | 48-114 |
| 11. | Toronto Blue Jays, 2009 | 114 | 48-114 |
| 12. | Montreal Expos, 1969 | 113 | 49-113 |
| 13. | Washington Senators, 1958 | 112 | 42-112 |
| 14. | Minnesota Twins, 1981 | 112 | 50-112 |
| 15. | Atlanta Braves, 1986 | 112 | 50-112 |
| 16. | New York Mets, 1967 | 111 | 51-111 |
| 17. | Milwaukee Brewers, 1971 | 111 | 51-111 |
| 18. | Seattle Pilots, 1969 | 110 | 52-110 |
| 19. | Chicago Cubs, 1970 | 110 | 52-110 |
| 20. | Milwaukee Brewers, 1975 | 110 | 52-110 |
| 21. | Seattle Mariners, 1977 | 110 | 52-110 |
| 22. | Toronto Blue Jays, 1978 | 110 | 52-110 |
| 23. | Montreal Expos, 1990 | 110 | 52-110 |
| 24. | Kansas City Royals, 1995 | 110 | 52-110 |
| 25. | New York Yankees, 1944 | 109 | 45-109 |
| 26. | Chicago White Sox, 1970 | 109 | 53-109 |
| 27. | Chicago White Sox, 1985 | 109 | 53-109 |
| 28. | Florida Marlins, 1993 | 109 | 53-109 |
| 29. | Cincinnati Reds, 2003 | 109 | 53-109 |
| 30. | Detroit Tigers, 2003 | 109 | 53-109 |
| 31. | Toronto Blue Jays, 2010 | 109 | 53-109 |
| 32. | New York Mets, 1963 | 108 | 54-108 |
| 33. | Atlanta Braves, 1974 | 108 | 54-108 |
| 34. | Detroit Tigers, 1976 | 108 | 54-108 |
| 35. | Chicago Cubs, 1978 | 108 | 54-108 |
| 36. | Chicago Cubs, 1983 | 108 | 54-108 |
| 37. | Montreal Expos, 1989 | 108 | 54-108 |
| 38. | Florida Marlins, 1998 | 108 | 54-108 |
| 39. | Toronto Blue Jays, 2001 | 108 | 54-108 |
| 40. | Colorado Rockies, 2002 | 108 | 54-108 |
| 41. | Washington Nationals, 2005 | 108 | 54-108 |
| 42. | San Diego Padres, 1969 | 107 | 55-107 |
| 43. | Montreal Expos, 1971 | 107 | 55-107 |
| 44. | Seattle Mariners, 1988 | 107 | 55-107 |
| 45. | Kansas City Royals, 2004 | 107 | 55-107 |
| 46. | Texas Rangers, 2008 | 107 | 55-107 |
| 47. | Philadelphia A's, 1946 | 106 | 48-106 |
| 48. | St. Louis Cardinals, 1956 | 106 | 48-106 |
| 49. | Washington Senators, 1956 | 106 | 48-106 |
| 50. | Chicago White Sox, 1980 | 106 | 56-106 |