| Rank | Team | Winning Percentage | Record |
| 1. | Seattle Mariners, 2010 | .2469 | 40-122 |
| 2. | Chicago Cubs, 1981 | .2531 | 41-121 |
| 3. | Seattle Mariners, 2005 | .2593 | 42-120 |
| 4. | Kansas City Royals, 1969 | .2716 | 44-118 |
| 5. | Washington Senators, 1958 | .2727 | 42-112 |
| 6. | Detroit Tigers, 1975 | .2901 | 47-115 |
| 7. | Oakland A's, 1977 | .2901 | 47-115 |
| 8. | Milwaukee Brewers, 2009 | .2901 | 47-115 |
| 9. | New York Yankees, 1944 | .2922 | 45-109 |
| 10. | Seattle Mariners, 1983 | .2963 | 48-114 |
| 11. | Washington Nationals, 2007 | .2963 | 48-114 |
| 12. | Washington Nationals, 2008 | .2963 | 48-114 |
| 13. | Toronto Blue Jays, 2009 | .2963 | 48-114 |
| 14. | Montreal Expos, 1969 | .3025 | 49-113 |
| 15. | Minnesota Twins, 1981 | .3086 | 50-112 |
| 16. | Atlanta Braves, 1986 | .3086 | 50-112 |
| 17. | Philadelphia A's, 1946 | .3117 | 48-106 |
| 18. | St. Louis Cardinals, 1956 | .3117 | 48-106 |
| 19. | Washington Senators, 1956 | .3117 | 48-106 |
| 20. | New York Mets, 1967 | .3148 | 51-111 |
| 21. | Milwaukee Brewers, 1971 | .3148 | 51-111 |
| 22. | Seattle Pilots, 1969 | .3210 | 52-110 |
| 23. | Chicago Cubs, 1970 | .3210 | 52-110 |
| 24. | Milwaukee Brewers, 1975 | .3210 | 52-110 |
| 25. | Seattle Mariners, 1977 | .3210 | 52-110 |
| 26. | Toronto Blue Jays, 1978 | .3210 | 52-110 |
| 27. | Montreal Expos, 1990 | .3210 | 52-110 |
| 28. | Kansas City Royals, 1995 | .3210 | 52-110 |
| 29. | Boston Braves, 1946 | .3247 | 50-104 |
| 30. | Chicago White Sox, 1970 | .3272 | 53-109 |
| 31. | Chicago White Sox, 1985 | .3272 | 53-109 |
| 32. | Florida Marlins, 1993 | .3272 | 53-109 |
| 33. | Cincinnati Reds, 2003 | .3272 | 53-109 |
| 34. | Detroit Tigers, 2003 | .3272 | 53-109 |
| 35. | Toronto Blue Jays, 2010 | .3272 | 53-109 |
| 36. | St. Louis Cardinals, 1940 | .3312 | 51-103 |
| 37. | Philadelphia A's, 1943 | .3312 | 51-103 |
| 38. | New York Mets, 1963 | .3333 | 54-108 |
| 39. | Atlanta Braves, 1974 | .3333 | 54-108 |
| 40. | Detroit Tigers, 1976 | .3333 | 54-108 |
| 41. | Chicago Cubs, 1978 | .3333 | 54-108 |
| 42. | Chicago Cubs, 1983 | .3333 | 54-108 |
| 43. | Montreal Expos, 1989 | .3333 | 54-108 |
| 44. | Florida Marlins, 1998 | .3333 | 54-108 |
| 45. | Toronto Blue Jays, 2001 | .3333 | 54-108 |
| 46. | Colorado Rockies, 2002 | .3333 | 54-108 |
| 47. | Washington Nationals, 2005 | .3333 | 54-108 |
| 48. | Chicago Cubs, 1933 | .3377 | 52-102 |
| 49. | Baltimore Orioles, 1959 | .3377 | 52-102 |
| 50. | San Diego Padres, 1969 | .3395 | 55-107 |